人结合蛋白2(TRAF2)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human TRAF2(TNF Receptor-Associated Factor 2) Microsample ELISA Kit","alternative_names":"TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3","species":"Human","assay_type":"Sandwich","sensitivity":"25 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRAF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRAF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRAF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRAF2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q12933","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8544MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8544MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8544MS",}