人肺表面活性蛋白D(SP-D)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human SP-D(Pulmonary surfactant-associated protein D) Microsample ELISA Kit","alternative_names":"SFTPD; SPD; SFTP 4;","species":"Human","assay_type":"Sandwich","sensitivity":"0.51 ng\/mL","standard":"80 ng\/mL","detection_range":"1.25-80 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Pulmonology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SP-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SP-D in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P35247","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8561MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8561MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8561MS",}