人肺表面活性蛋白D(SP-D)微量上样酶联免疫吸附检测试剂盒
- 货号：ELK8561MS
- 价格：￥2,400.00 元
- 点击：1445
产品介绍
产品信息
| 英文名称 | Human SP-D(Pulmonary surfactant-associated protein D) Microsample ELISA Kit |
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| 别名 | SFTPD; SPD; SFTP 4; |
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规格与价格
| 48T 价格 | 1680 |
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| 96T 价格 | 2400 |
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| 96T*5 价格 | 10200 |
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检测参数
| 种属 | Human |
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| 检测类型 | Sandwich |
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| 灵敏度 | 0.51 ng/mL |
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| 检测范围 | 1.25-80 ng/mL |
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| 样本类型 | serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Infection immunity;Pulmonology; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SP-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SP-D in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt编号 | P35247 |
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标准曲线
| 标准曲线图 |  |
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