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人凝血酶原前体蛋白(PIVKA-II)酶联免疫吸附检测试剂盒

  • 货号:ELK8570
  • 价格:¥2,600.00 元
  • 点击:481

产品介绍

产品信息

英文名称Human PIVKA-II(Protein Induced by Vitamin K Absence or Antagonist-II) ELISA Kit
别名PIVKA-II;  PIVKA II

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.129 ng/mL
检测范围0.32-20 ng/mL
样本类型serum, plasma and other biological fluids
实验时长3.5h

研究信息

研究领域Hematology
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIVKA-II. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIVKA-II. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIVKA-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIVKA-II in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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