人癌抗原27-29 (CA 27-29)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human CA 27-29(cancer antigen 27-29) Microsample ELISA Kit","alternative_names":"CA 27-29","species":"Human","assay_type":"Sandwich","sensitivity":"0.8 U\/mL","standard":"100 U\/mL","detection_range":"1.57-100 U\/mL","sample_type":"Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.","assay_length":"3.5h","research_area":"Cancer","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8602MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8602MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8602MS",}