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牛Ⅲ型前胶原氨基端原肽(PIIINP)酶联免疫吸附检测试剂盒

  • 货号:ELK8757
  • 价格:¥2,800.00 元
  • 点击:733

产品介绍

产品信息

英文名称Cattle PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
别名P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Cattle
检测类型Sandwich
灵敏度2.29 ng/mL
检测范围6.25-400 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Metabolic pathway;Hepatology;Bone metabolism;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PIIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PIIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PIIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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