牛诱导型一氧化氮合成酶(NOS2/iNOS)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Cattle NOS2\/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit","alternative_names":"NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible","species":"Cattle","assay_type":"Sandwich","sensitivity":"0.124 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NOS2\/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NOS2\/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NOS2\/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NOS2\/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q27995","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8803-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8803-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8803",}