人P21激活酶1(PAK1)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PAK1(p21 activated kinase1) Microsample ELISA Kit","alternative_names":"Alpha PAK; p21 activated kinase 1; p65 PAK; PAK 1; PAK1; PAKalpha","species":"Human","assay_type":"Sandwich","sensitivity":"6 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;Metabolic pathway;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAK1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q13153","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8847MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8847MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8847MS",}