小鼠趋化因子C-C-基元配体6(CCL6)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse CCL6(Chemokine C-C-Motif Ligand 6) Microsample ELISA Kit","alternative_names":"Ccl6C-C motif chemokine 6 ; Small-inducible cytokine A6","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.063 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Cytokine;Tumor immunity;Infection immunity;Hepatology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CCL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CCL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CCL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CCL6 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P27784","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8887MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8887MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8887MS",}