大鼠含PYD和CARD域蛋白(PYCARD)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit","alternative_names":"ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1","species":"Rat","assay_type":"Sandwich","sensitivity":"7.4 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Apoptosis;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PYCARD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PYCARD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PYCARD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PYCARD in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8931-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8931-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8931",}