| 英文名称 | Rat VD3(Vitamin D3) ELISA Kit |
|---|---|
| 别名 | Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol |
| 48T 价格 | 1960 |
|---|---|
| 96T 价格 | 2800 |
| 96T*5 价格 | 11900 |
| 种属 | Rat |
|---|---|
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 2.11 ng/mL |
| 检测范围 | 6.25-400 ng/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 实验时长 | 2h |
| 研究领域 | Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism; |
|---|---|
| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat VD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VD3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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