人叉头状蛋白N1(FOXN1)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human FoxN1(Forkhead box N1) Microsample ELISA Kit","alternative_names":"Forkhead box N1; FKHL20; Forkhead box protein N1; FOXN 1; FOXN1; Transcription factor winged-helix nude","species":"Human","assay_type":"Sandwich","sensitivity":"6.6 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Immunology","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FoxN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FoxN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FoxN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FoxN1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O15353","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9113MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9113MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9113MS",}