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人Ⅰ类主要组织相容性复合体F(MHCF)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK9133MS
  • 价格:¥2,600.00 元
  • 点击:2021
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产品介绍

产品信息

英文名称Human MHCF(Major Histocompatibility Complex Class I F) Microsample ELISA Kit
别名CDA12; HLA 5.4; HLA CDA12; HLA F; HLA F antigen; HLAF; HLA-F; Leukocyte antigen F; MHC class I antigen F

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度5.2 pg/mL
检测范围15.63-1000 pg/mL
样本类型Serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P30511

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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