绵羊性激素结合球蛋白(SHBG)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Sheep SHBG(Sex Hormone Binding Globulin) ELISA Kit","alternative_names":"ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin","species":"Sheep","assay_type":"Sandwich","sensitivity":"0.59 ng\/mL","standard":"100 ng\/mL","detection_range":"1.57-100 ng\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Endocrinology;Reproductive science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep SHBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep SHBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep SHBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep SHBG in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9135-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9135-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9135",}