{"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Chicken IgY(Immunoglobulin of Yolk) ELISA Kit","alternative_names":"Immunoglobulin Yolk","species":"Chicken","assay_type":"Competitive Inhibition","sensitivity":"1.81 µg\/mL","standard":"400 µg\/mL","detection_range":"6.25-400 µg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"2h","research_area":"Infection immunity;Immune molecule;Hematology;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken IgY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IgY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IgY in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9182-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9182-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9182",}