大鼠X-连锁凋亡蛋白抑制因子(XIAP)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat XIAP(Inhibitor Of Apoptosis Protein, X-Linked) ELISA Kit","alternative_names":"BIRC4; API3; ILP1; MIHA; XLP2; Baculoviral IAP Repeat-Containing 4; E3 ubiquitin-protein ligase XIAP; IAP-like; Inhibitor of apoptosis protein 3; X-linked inhibitor of apoptosis protein","species":"Rat","assay_type":"Sandwich","sensitivity":"0.057 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Apoptosis;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat XIAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat XIAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat XIAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat XIAP in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9R0I6","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9200-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9200-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9200",}