小鼠信号素4A(SEMA4A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse SEMA4A(Semaphorin 4A) ELISA Kit","alternative_names":"CORD10; RP35; SEMAB; SEMB; Semaphorin-B; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4A","species":"Mouse","assay_type":"Sandwich","sensitivity":"13.4 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"CD & Adhesion molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SEMA4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SEMA4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SEMA4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SEMA4A in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q62178","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9296-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9296-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9296",}