人磷酸化神经丝蛋白H(pNF-H)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit","alternative_names":"TC2; Transcobalamin II","species":"Human","assay_type":"Sandwich","sensitivity":"29 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Neuro science","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9302-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9302-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9302",}