大鼠可溶性fms样酪氨酸激酶1(sFLT)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Rat sFLT(Soluble Fms-Like Tyrosine Kinase Receptor 1) ELISA Kit","alternative_names":"Flt1; Fms-Related Tyrosine Kinase 1; Vascular Permeability Factor Receptor; Fms-like tyrosine kinase 1; Tyrosine-protein kinase receptor FLT","species":"Rat","assay_type":"Sandwich","sensitivity":"29 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sFLT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sFLT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sFLT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sFLT in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9331-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9331-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9331",}