大鼠高迁移率族AT Hook蛋白1(HMGA1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat HMGA1(High Mobility Group AT Hook Protein 1) ELISA Kit","alternative_names":"HMG-R; HMGIY; High mobility group protein A1; High mobility group protein R; High mobility group protein HMG-I\/HMG-Y","species":"Rat","assay_type":"Sandwich","sensitivity":"0.115 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;Metabolic pathway;Tumor immunity;Developmental science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGA1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q8K585","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9443-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9443-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9443",}