人高亲和力低亲和力神经生长因子受体(TRKA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human TrkA\/NTRK1(High affinity nerve growth factor receptor) ELISA Kit","alternative_names":"NTRK1; MTC; TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A;","species":"Human","assay_type":"Sandwich","sensitivity":"32 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Cell Biology; Neuroscience; Signal Transduction; Developmental Biology","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrkA\/NTRK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrkA\/NTRK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrkA\/NTRK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrkA\/NTRK1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P04629","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9487-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9487-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9487",}