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大鼠白细胞分化抗原CD4(CD4)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK9500MS
  • 价格:¥2,400.00 元
  • 点击:977

产品介绍

产品信息

英文名称Rat CD4(Cluster Of Differentiation 4) Microsample ELISA Kit
别名CD4mut; p55; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4; Cluster Of Differentiation 4

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Rat
检测类型Sandwich
灵敏度38 pg/mL
检测范围78.13-5000 pg/mL
样本类型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;CD & Adhesion molecule;Immune molecule;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P05540

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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