| 英文名称 | F-Testo(Free Testosterone) ELISA Kit |
|---|---|
| 别名 | F-TESTO |
| 48T 价格 | 1680 |
|---|---|
| 96T 价格 | 2400 |
| 96T*5 价格 | 10200 |
| 种属 | General |
|---|---|
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 18.76 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| 实验时长 | 2h |
| 研究领域 | Endocrinology;Reproductive science;Hormone metabolism; |
|---|---|
| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-TESTO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-TESTO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-TESTO in the samples is then determined by comparing the OD of the samples to the standard curve. |