小鼠D-乳酸脱氢酶(D-LDH)酶联免疫吸附检测试剂盒
- 货号：ELK9609
- 价格：￥2,400.00 元
- 点击：985
产品介绍
产品信息
| 英文名称 | Mouse D-LDH(D-LactateDehydrogenase) ELISA Kit |
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| 别名 | DLD; LDHD; Probable D-lactate dehydrogenase;mitochondrial; |
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规格与价格
| 48T 价格 | 1680 |
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| 96T 价格 | 2400 |
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| 96T*5 价格 | 10200 |
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检测参数
| 种属 | Mouse |
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| 检测类型 | Sandwich |
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| 灵敏度 | 2.74 mIU/mL |
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| 检测范围 | 4.69-300 mIU/mL |
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| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Signal Transduction;Cancer;Kits/ Lysates/ Other;Metabolism |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse D-LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse D-LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse D-LDH in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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