小鼠白介素27(IL-27)微量上样酶联免疫吸附检测试剂盒

英文名称	Mouse IL-27(Interleukin 27) Microsample ELISA Kit
别名	IL-27; IL-27A; IL27p28; IL30; MGC71873; p28;

种属	Mouse
检测类型	Sandwich
灵敏度	19.12 pg/mL
检测范围	31.25-2000 pg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Immunology;Cancer
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q8K3I6

标准曲线图

【仅供科学研究使用】

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