大鼠前列腺素F2α(PGF2a)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat PGF2α(Prostaglandin F2 Alpha) ELISA Kit","alternative_names":"PGF2A; PGF2-A; PG-F2a","species":"Rat","assay_type":"Competitive Inhibition","sensitivity":"3.71 pg\/mL","standard":"800 pg\/mL","detection_range":"12.5-800 pg\/mL","sample_type":"Serum, plasma, saliva and other biological fluids","assay_length":"2h","research_area":"Metabolic pathway;Cardiovascular biology;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9878-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9878-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9878",}