小鼠免疫球蛋白D(IgD)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse IgD (Immunoglobulin D) ELISA Kit","alternative_names":"IGHD; Constant Region Of Heavy Chain Of Immunoglobulin Delta","species":"Mouse","assay_type":"Sandwich","sensitivity":"6.9 ng\/mL","standard":"1000 ng\/mL","detection_range":"15.63-1000 ng\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Immune molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IgD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IgD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgD in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK9888-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK9888-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK9888",}