| Immunocytochemistry/ Immunofluorescence | ICCIF | Human | 已验证 | 1/100 - 1/1000 | Fix cells in ice-cold MeOH |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 不推荐 | 1/100 - 1/1000 | High background was observed in images. |
| Western blot | WB | Human | 已验证 | 1/500 - 1/3000 | 5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight. Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Mouse | 保证反应 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Mouse | 不推荐 | 1/100 - 1/1000 | High background was observed in images. |
| Western blot | WB | Mouse | 已验证 | 1/500 - 1/3000 | 5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight. Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
| Immunocytochemistry/ Immunofluorescence | ICCIF | Rat | 保证反应 | | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Rat | 不推荐 | | |
| Western blot | WB | Rat | 已验证 | 1/500 - 1/3000 | 5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight. Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
