| 中文名称 | Anti-EGF 抗体 |
|---|---|
| 英文名称 | Anti-EGF antibody [Lyophilized] |
| 别名 | Pro-epidermal growth factor、EGF |
| 靶点符号 | EGF |
| 产品类型 | 一抗 |
| 宿主种属 | Rabbit |
|---|---|
| 克隆 | Polyclonal |
| 亚型 | IgG |
| 轻链类型 | unknown |
| 不含载体蛋白 | No |
| 反应种属 | Human |
| 应用 | WB、IHC-P、ELISA、sELISA |
| 免疫原 | Recombinant Full Length Protein corresponding to Human EGF.;P01133 |
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| ELISA | ELISA | Human | 保证反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 保证反应 | ||
| Western blot | WB | Human | 保证反应 | ||
| Sandwich ELISA | sELISA | Human | 保证反应 | ||
| ELISA | ELISA | Recombinant full length protein - Human | 保证反应 | To detect hEGF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF. | |
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Recombinant full length protein - Human | 不推荐 | ||
| Western blot | WB | Recombinant full length protein - Human | 已验证 | 0.1-0.2 µg/mL | To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa. |
| Sandwich ELISA | sELISA | Recombinant full length protein - Human | 已验证 | 0.5-2 µg/mL | For sandwich ELISA, use this antibody as Detection at 0.5μg/ml with as Capture. |
| 形式 | Lyophilized |
|---|---|
| 纯化工艺 | Affinity purification Immunogen |
| 推荐的短期储存时间 | 1-2 weeks |
| 复溶 | Reconstitute with 200µl of sterile water. |