| 中文名称 | Anti-IL-1 beta 抗体(RB9A37060294) |
|---|---|
| 英文名称 | Anti-IL-1 beta antibody (RB9A37060294) |
| 别名 | IL1F2、IL1B、Interleukin-1 beta、IL-1 beta、Catabolin |
| 靶点符号 | IL1B |
| 产品类型 | 一抗 |
| 宿主种属 | Rabbit |
|---|---|
| 克隆 | Polyclonal |
| 亚型 | Unknown |
| 轻链类型 | unknown |
| 不含载体蛋白 | No |
| 反应种属 | Human |
| 应用 | sELISA、IHC-P、WB |
| 免疫原 | Full Length Protein corresponding to Mouse Il1b aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.;P10749 |
| 特异性 | Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta. |
| 应用 | 应用代码 | 物种 | 验证状态 | 推荐稀释 | 备注 |
|---|---|---|---|---|---|
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Human | 已验证 | 2 µg/mL | Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
| Western blot | WB | Human | 保证反应 | ||
| Sandwich ELISA | sELISA | Human | 保证反应 | ||
| Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) | IHCP | Recombinant full length protein - Mouse | 不推荐 | ||
| Western blot | WB | Recombinant full length protein - Mouse | 已验证 | 0.1-0.2 µg/mL | Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich ELISA | sELISA | Recombinant full length protein - Mouse | 已验证 | 2 µg/mL | To detect mIL-1b by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b. |
| 形式 | Lyophilized |
|---|---|
| 纯化工艺 | Affinity purification Immunogen |
| 推荐的短期储存时间 | 1-2 weeks |
| 储存信息 | Avoid freeze / thaw cycle |
| 复溶 | Reconstitute in water |