当前位置:首页>产品中心>单抗/多抗>Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody|抗VEEV刺突糖蛋白E2多克隆抗体(靶标:委内瑞拉马脑炎病毒刺突糖蛋白E2)

Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody|抗VEEV刺突糖蛋白E2多克隆抗体(靶标:委内瑞拉马脑炎病毒刺突糖蛋白E2)

  • 货号:RB9TVK039024
  • 价格:面议
  • 点击:167

产品介绍

产品参数

形态Liquid
宿主种属Rabbit
克隆性Polyclonal
抗体亚型IgG
纯化方式Purified by antigen affinity column.
内毒素水平Please contact with the lab for this information.
应用ELISA, IHC, WB
种属反应性Venezuelan equine encephalitis virus
靶标6K protein, Assembly protein E3, C, Capsid protein, Coat protein, E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotei
免疫原E. coli - derived recombinant VEEV Structural polyprotein (Ser335-Pro679).
储存缓冲液0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
稳定性和储存Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
产品使用说明ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000
登录号P09592
背景信息

Structural polyprotein (E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Garmashova, N. et al. (2007) Journal of virology 81, 2472-84. PMID: 17108023
2. Garmashova, N. et al. (2007) Journal of virology 81, 13552-65. PMID: 17913819
3. Atasheva, S. et al. (2010) Journal of virology 84, 4158-71. PMID: 20147401
4. Carey, BD. et al. (2018) Journal of virology 92. PMID: 29769351
备注For research use only
【仅供科学研究使用】

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