Recombinant Human/Cynomolgus CD28 (C-Fc)

产品介绍

{"gui_ge":"10ug","biao_da_su_zhu":"Human Cells","biao_qian":"C-Fc","fen_zi_liang":"42.3 KDa","biao_guan_fen_zi_liang":"60 KDa, reducing conditions","xing_tai":"Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.","chun_du":"Greater than 95% as determined by reducing SDS-PAGE.","nei_du_su_shui_ping":"Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.","huo_xing":"Immobilized Anti-CD28 mAb(Cat#GMP-A063) at 2μg\/ml (100 μl\/well) can bind Human\/Cynomolgus CD28-Fc *(Cat#CX81)*: Biotinylated by NHS-biotin prior to testing The ED50 of Human\/Cynomolgus CD28-Fc *(Cat#CX81) is 83.5 ng\/ml.","rong_jie_fang_fa":"Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. ","chu_cun_fang_fa":"Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at < -20°C for 3 months.","yun_shu_tiao_jian":"The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.","bei_jing_shuo_ming":"T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells."}