犬前列腺素F代谢物(PGFM)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Dog PGFM(Prostaglandin F Metabolite) ELISA Kit","alternative_names":"13,14,Dihydro-15-keto-Prostaglandin F2伪","species":"Dog","assay_type":"Sandwich","sensitivity":"4 pg\/mL","standard":"600 pg\/mL","detection_range":"9.38-600 pg\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog PGFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog PGFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog PGFM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog PGFM in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK0656-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK0656-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK0656",}