人上皮型钙黏蛋白(E-Cad)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human E-Cad(E-Cadherin) Microsample ELISA Kit","alternative_names":"CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial","species":"Human","assay_type":"Sandwich","sensitivity":"0.11 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"serum, plasma, urine, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"CD & Adhesion molecule;Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P12830","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK11056MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK11056MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK11056MS",}