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人上皮型钙黏蛋白(E-Cad)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK11056MS
  • 价格:¥2,400.00 元
  • 点击:1786

产品介绍

产品信息

英文名称Human E-Cad(E-Cadherin) Microsample ELISA Kit
别名CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Human
检测类型Sandwich
灵敏度0.11 ng/mL
检测范围0.32-20 ng/mL
样本类型serum, plasma, urine, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域CD & Adhesion molecule;Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P12830

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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