人肠脂肪酸结合蛋白(IFABP/FABP2)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human IFABP\/FABP2(Intestinal Fatty Acid Binding Protein) Microsample ELISA Kit","alternative_names":"FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal","species":"Human","assay_type":"Sandwich","sensitivity":"0.06 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFABP\/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFABP\/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFABP\/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFABP\/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P12104","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1264MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1264MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1264MS",}