小鼠诱导型一氧化氮合成酶(NOS2/iNOS)酶联免疫吸附检测试剂盒

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• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse NOS2\/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit","alternative_names":"NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.116 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2\/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NOS2\/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NOS2\/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NOS2\/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P29477","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK1504-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK1504-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK1504",}