人CD30配体(CD30L)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human CD30L(Cluster Of Differentiation 30 Ligand) Microsample ELISA Kit","alternative_names":"CD153; TNFSF8; CD30-L; Tumor Necrosis Factor Ligand Superfamily Member 8","species":"Human","assay_type":"Sandwich","sensitivity":"33 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD30L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD30L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD30L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD30L in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P32971","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2306MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2306MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2306MS",}