小鼠全段甲状旁腺素(I-PTH)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse I-PTH(IntactParathormone) Microsample ELISA Kit","alternative_names":"iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone","species":"Mouse","assay_type":"Sandwich","sensitivity":"3.96 pg\/mL","standard":"800 pg\/mL","detection_range":"12.5-800 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;Endocrinology;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2564MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2564MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2564MS",}