小鼠蛋白酪氨酸磷酸酶样蛋白A(PTPLA)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Mouse PTPLA(Protein Tyrosine Phosphatase Like Protein A) ELISA Kit","alternative_names":"CAP; HACD1; Proline Instead Of Catalytic Arginine Member a; 3-hydroxyacyl-CoA dehydratase 1; Cementum Attachment Protein; Very-long-chain 3R-3-hydroxyacyl-CoA dehydratase 1","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.109 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTPLA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTPLA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PTPLA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PTPLA in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9QY80","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK7812-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK7812-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK7812",}