烟酸酰胺腺嘌呤二核苷酸磷酸(NAADP)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit","alternative_names":"NAADP","species":"General","assay_type":"Competitive Inhibition","sensitivity":"51 ng\/mL","standard":"10000 ng\/mL","detection_range":"156.25-10000 ng\/mL","sample_type":"serum, plasma, cell lysates, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8020-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8020-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8020",}