8-异构前列腺素F2α(8-epi-PGF2a)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"8-epi-PGF2a(8-Epi Prostaglandin F2 Alpha) ELISA Kit","alternative_names":"8-EpiPGF2-α; 8epi-PGF2a","species":"General","assay_type":"Competitive Inhibition","sensitivity":"9.23 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma and other biological fluids","assay_length":"2h","research_area":"Endocrinology;Pulmonology;Hepatology;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-epi-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-epi-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK8044-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK8044-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK8044",}