3C Protease|3C蛋白酶(靶标：3C蛋白酶（来源于肠道病毒）)
- 货号：RB9TVK794012
- 价格：面议
- 点击：523
产品介绍
产品参数
| 产品描述 | 3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.<br>Activity definition: 1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 μg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C. |
|---|---|
| 形态 | Liquid |
| 性质 | Recombinant |
| 纯度 | >95% as determined by SDS-PAGE. |
| 内毒素水平 | Please contact with the lab for this information. |
| 应用 | Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP). |
| 储存缓冲液 | 50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol |
| 稳定性和储存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| 重构方法 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 运输条件 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 表达系统 | E. coli |
| 蛋白长度 | 3C Protease is cloned from Human rhinovirus, fused |
| 预测分子量 | 47.46 kDa |
| 种属 | HRV-14 |
| 备注 | For research use only. |
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