Anti-Human TRIM65 Polyclonal Antibody|抗人TRIM65多克隆抗体(靶标：人三联基序蛋白65)
- 货号：RB9THD347014
- 价格：面议
- 点击：425
产品介绍
产品参数
| 形态 | Liquid |
|---|---|
| 宿主种属 | Rabbit |
| 克隆性 | Polyclonal |
| 抗体亚型 | IgG |
| 纯化方式 | Purified by antigen affinity column. |
| 内毒素水平 | Please contact with the lab for this information. |
| 应用 | ELISA, IHC, WB |
| 种属反应性 | Human |
| 靶标 | E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, TRIM65, Tripartite motif-containing protein 65 |
| 免疫原 | E. coli - derived recombinant Human TRIM65 (Val319-Ser517). |
| 储存缓冲液 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| 产品使用说明 | ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000 |
| 登录号 | Q6PJ69 |
| 背景信息 | E3 ubiquitin-protein ligase TRIM65 is a ~57 kDa protein. E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation. Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs. This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy. Upon enteroviral infection, promotes 'Lys-63'-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade. Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs. 1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402 2. Tang, T. et al. (2021) Frontiers in immunology 12, 741839. PMID: 34512673 3. Li, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 6970-5. PMID: 24778252 4. Pan, X. et al. (2019) Cell death & disease 10, 429. PMID: 31160576 5. Kato, K. et al. (2021) Molecular cell 81, 599-613.e8. PMID: 33373584 6. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649 |
| 备注 | For research use only |
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